The yeast two-hybrid (Y2H) system (Figure 1) can be used to screen for (novel) protein–protein interactions. Physical binding between a protein of interest (the bait) and proteins of a library (the preys) can be detected. When using sophisticated strategies / systems, relatively easy millions of ‘library prey’s’ can be tested to see whether they bind to the bait protein of interest in the Y2H system. ♦♦♦ 5 Y2H screens starting from around €8000 (ex. tax and analysis of positive clones, for details see Table 1). ♦♦♦
The yeast two-hybrid (Y2H) system / assay (Figure 1) is a well-known technique that can be used to demonstrate physical binding between two proteins of interest experimentally. The system is often very suitable to verify putative / potential protein–protein interactions (PPIs) that were identified using affinity purification-mass spectrometry (AP-MS) or any other method to identify PPIs, including PPI prediction methods. Once a PPI has been confirmed in the Y2H system, involved binding sites can be mapped relatively easily (lean more). Once a binding site has been identified, potential peptide protein–protein interaction inhibitors can be derived (learn more). See Table 1 to learn about our protein–protein interaction testing service (Cat. No. C200).
A short linear peptide that matches (a part of) a specific protein–protein interaction domain (PPID) can be used as a tool that binds to a corresponding interacting / complementary PPID of an endogenous protein when added to cells in culture. As a consequence of that binding, protein–protein interaction (PPI) function of the targeted PPID will be prevented specifically, and as a consequence of that prevention, the targeted cellular process of interest can be disrupted. ♦♦♦ See also the above slideshow. ♦♦♦