The yeast two-hybrid (Y2H) system (Figure 1) can be used to screen for (novel) protein–protein interactions. Physical binding between a protein of interest (the bait) and proteins of a library (the preys) can be detected. When using sophisticated strategies / systems, relatively easy millions of ‘library prey’s’ can be tested to see whether they bind to the bait protein of interest in the Y2H system.
A short linear peptide that matches (a part of) a specific protein–protein interaction domain (PPID) can be used as a tool that binds to a corresponding interacting / complementary PPID of an endogenous protein when added to cells in culture. As a consequence of that binding, protein–protein interaction (PPI) function of the targeted PPID will be prevented specifically, and as a consequence of that prevention, the targeted cellular process of interest can be disrupted.